Primeroni Peperoni
Evaluate primer quality by their thermodynamic properties and structural features
Primer 1 (Forward, 5' to 3')
Primer 2 (Reverse, 5' to 3') (Optional)
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Essential Parameters
Na⁺ (mM)
Mg²⁺ (mM)
dNTP (mM total)
Primer Conc (nM)
Annealing Temp (°C)
Advanced Settings (Tier 2)
K⁺ (mM)
Tris (mM)
Tm / Salt Cor. Formula
Owczarzy 2008 (Auto)
SantaLucia 1998 + Na eq
Eval Temp (°C)
Max Hairpin Size
Thresholds (Tier 3)
Max Hairpin ΔG
Max Dimer Global ΔG
Max Dimer 3' Extensible ΔG
Max ΔTm (°C)
Max 3' Stability ΔG
Analyze Primers
Primer
Score
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Penalties
Tm
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GC%
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Length
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Target ΔG
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Secondary Structures
Sequence Checks